J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (3): -.

• Research Articles •    

The Cloning of rolC Gene and Over Expression of Cytokinins in Nicotiana tabacum

JIA Yan-Tao, MA Mi, QU Gui-Ping, QIAN Zhong-Xing and LIN Zhong-Ping   

Abstract: Using PCR method the rolC gene was amplified from Agrobacterium rhizogenes, and CaMV 35S/rolC expression vector pCaR was constructed. The chimeric gene via agrobacterium mediated procedure was transformed separately into the wild type tobacco (Nicotiana tabacum L. cv. W38) and the transgenic tobacco of ipt gene. The putative transgenic plants were assayed with Southem blot and RNA Dot blot analysis. The observation suggested that the transgenic tobacco exhibited the abnormal phenotypes as a consequence of the overproduction of cytokinins. Whereas the ELISA assay indicated that the cytokinins level increased separately in transgenic plants. The growth of the transgenic plants show multiple budding of shoots with short intemodal length.

Key words: rolC gene, Cytokinin, Tobacco

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