J Integr Plant Biol. ›› 1998, Vol. 40 ›› Issue (2): -.

• Research Articles •    

Recent Advances in in vitro Fertilization and Zygote Culture of Angiosperms

YANG Hong-Yuan and ZHOU Chang   

Abstract: The successful in vitro fertilization and zygote culture in angiosperms is the most important breakthrough in the experimental researches on sexual plant reproduction during recent years. The present review includes the following parts: 1 ) A brief survey on the historical backgrounds and technical prerequisites of the achievements. 2) Progresses of manipulation techniques, including methods of egg cell isolation, intergametic fusion, microculture of artificial as well as natural zygotes and regeneration of plants, in vitro development from fusion products between gametes of alien species, from unfertilized egg cells and from central cells, etc. 3) Cytobiological and molecular biological studies on in vitro fertilization systems, e. g. time table of developmental events during in vitro fertilization and postfertilization processes, cell wall formation of artificial zygotes in relation to polyspermy preventation, egg cell activation, zygote polarity and asymmetric division of zygotes, construction of cDNA libraries from egg cells, zygotes and early embryos using RT/PCR technique and screening of specific genes, and transient expression of reporter genes introduced into isolated zygotes. 4) Strategies for further broadening and deepening the researches in this area beth in fundamental and applied aspects. It is hopeful that in vitro fertilization and zygote culture will bring new lights in the developmental biology of fertilization and early embryogenesis and open up new prospects for biotechnology in plants.

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