J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (12): -.

• Research Articles •    

Interspecific Somatic Hybrids in Actinidia

Xiao Zun-an and Han Bi-wen   

Abstract: Protoplasts isolated from cotyledon-derived calli of Actinidia chinensis var. chinensis (2n = 2x = 58) were fused by the PEG method with cotyledon-callus protoplasts of A. deliciosa var. deliciosa (2n = 6x = 174) or with mesophyll protoplasts of A. kolomikta (2n = 2x = 58), respectively. Randomly amplified polymorphic DNA (RAPD) markers and flow cytometry was used to confinn the occurrence of somatic hybrids. RAPD results with some primers surveyed indicated that one clone (A. chinensis + A. deliciosa ) and four clones (A. chinensis + A. kolomikta ) had RAPD banding patterns which combined the parental banding profiles. Ploidy levels of the (A. chinensis + A. deliciosa ) clone were deduced as octoploid (2n = 8x), and the (A. chinensis + A. kolomikta) clones were tetraploid (2n = 4x), triploid (2n = 3x) or pentaploid (2n= 5x). The clones were confirmed as interspecific somatic hybrids in Actinidia.

Key words: Actinidia chinensis, Actinidia deliciosa, Actinidia kolomikta, interspecific somatic hybrid

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