J Integr Plant Biol. ›› 1997, Vol. 39 ›› Issue (8): -.

• Research Articles •    

GUS Activities Under Control of HRGP Promoter of Carrot

Yin Shu-ping, Guo Yang, Ma Mi, Hu Yuan-lei, Zhao Wei-ping and Lin Zhong-ping   

Abstract: Hydroxyproline rich glycoprotein (HRGP) promoter has been cloned from genomic clone of carrot ( Daucus carom var. sativa DC. ) by PCR procedure. The construction with fusion gene of carrot HRGP/GUS in binary vector were used for the genetic transformation by Agrobacter/um mediated procedure and the transgenie tobaccoes with this transgene were obtained. The GUS activities in various organs have been studied by histochemical examination. Tissue specific activities of this HRGP promoter were demonstrated. GUS activities were expressed in the apical meristematic zones of roots, inner phloem of stems, inner and outer phloem of petioles. The highest levels of GUS activity were found in tissues where axillary bud was initiated. The expression of GUS activities under the control of carrot HRGP promoter could also be enhanced by treatment with the inducing factors such as salicylic acid, CaC12 and NaC1.

Key words: Carrot, Hydroxyproline rich glycoproteins promoter, GUS, Transgenic tobacco

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