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J Integr Plant Biol, 1990, 32 (12): -, Research Article
Plant Regeneration from Sainfoin(Onobrychis viciaefolia)Protoplasts Derived from Cell Suspensions of Hypocotyl
Zhao Gui-lan
The protoplasts were isolated from cell suspension cultures of hypocotyl (Onobrychis viciaefolia) cullured continuously for 3–4 months, and were cultured in modified Wguid Ⅴ- KM medium. The first division of the regenerated cell occurred after 24 h. culture. Small calli could be seen with naked eyes in 4 weeks. The calli which were propagated to 2–4 mm long in diameter in the (Ⅳ) medium were transferred onto differentiation medium and shoots appeared after 2–3 weeks. The differentiated shoots formed their roots on 1/2 MS supplamented with NAA 1.0mg/1 and grew into plantlets.
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