J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (8): -.

• Research Articles •    

Protoplasts Culture and Plant Regeneration of Soybean (Glycine max L. and G. soja)

Wei Zhi-ming and Xu Zhi-hong   

Abstract: Protoplasts were isolated enzymatically from immature cotyledons of soybean. The protoplasts divided to form calli in the K8P liquid medium. The calli further grew to 2–3 mm on the solid K8 medium and were transferred onto the MSB medium (MS minerals+B5 organic components+0.5–1.0 mg/l 2,4-D+0.2–0.5 mg/l BA) to obtain compact and nodular calli. Shoot formation was initiated on M1 medium (MSB medium with 0.15 mg/1 NAA, and BA, KT and ZT, 0.5 mg/l of each, 500 mg/1 CH). Differentiation frequency was 13.6–24.2%. Plants have been regenerated from protoplasts of immature cotyledons in 2 cultivars, and normal pods were obtained from them.

Key words: Soybean (Glycine max L.), Protoplasts, Plant regeneration

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