J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (1): -.

• Research Articles •    

Selection and Characterization of Asparagus Mutants Resistant to Inhibition by Lysine Plus Threonine

Yin Yan-hai, Chen Hui, Kuang Bai-jian and Wang Jing-ju   

Abstract: Asparagus officinalis calli were induced from shoot of seedlings. After mutagenization, two lysine plus threonine resistant mutant lines (LTR2, LTR3) were obtained by selectionnonselection-reselection procedures with 2 mmol/1 lysine plus threonine. LTR2 and LTR3 caIli remained resistance to lysine plus threonine after being subcultured for 1 year, and both of them showed cross resistance to 1 mmol/l aminoethylcysteine. In resistant calli, the free lysine, methionine and an unknown amino acid were l-l0 times more than those in controls.

Key words: Asparagus officinalis, Lysine plus threonine, Resistant calli

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