J Integr Plant Biol. ›› 1990, Vol. 32 ›› Issue (1): -.

• Research Articles •    

Partial cDNA Cloning and Nucleotide Sequence of Rice Dwarf Virus Genome

Gao Qian, Ou Yang-xin, Liu Wei, Pan Nai-sui and Chen Zhang-liang   

Abstract: Rice dwarf virus (RDV) was isolated and purified from infected rice leaves with chloro form extraction, PEG precipitation and sucrose gradient centrifugation. Total RDV RNA ge nome was separated in the agarose gel and segments of RDV RNA genome were purified. The cDNAs of several segments were synthesized with oligo dT as primer. Through cDNA mapping, subcloning and sequencing, we have obtained partial DNA sequence of those segments. Here we report the cloning and partial DNA sequence of segment 8 from RDV RNA genome.

Key words: Rice Dwarf Virus, RDV cDNA clone, RDV cDNA sequerce

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