J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (10): -.
• Research Articles •
Huang Ju-fu, Luo Ai-ling, Xie Xue-mei and Wang Ao-jin
After the exposure to air, the crystalline nitrogenase MoFe protein from Azotobacter vinelandii was resulted in the remarkable increase in its absorption (ABS) and the significant decrease in its activity and circular dichroism (CD). However, when the aerated MoFe protein was incubated with the reconstituting solution which consisted of Na2MoO4, ferric citrate, Na2S and dithiothreitol, the ABS and CD of the aerated. MoFe protein both were completely restored, simultaneously with the significant restoration of acetylene reduction. It is shown that the P-cluster and other parts related to the protein activity which was damaged by O2 are able to be repaired to a certain extent by the reconstituting solution.
Circular dichroism spectrum,
Aerated MoFe protein,
Nitrogenase from Azotobacter vinelandii
Huang Ju-fu, Luo Ai-ling, Xie Xue-mei and Wang Ao-jin. Studies on the Reactivation of Aerated Nitrogenase MoFe Protein from Azotobacter vinelandii by the Compounds of iron Molybdenum and Sulfur[J]. J Integr Plant Biol., 1989, 31(10): -.
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