J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (1): -.

• Research Articles •    

Plant Regeneration from Cotyledon Protoplasts of Tomato (Lycopersicon esculentum L.)

Wang Guang-yuan, Xia Zhen-ao (Hsia Chen-au) , Chi Jing-fen   

Abstract: otyledon protoplasts of tomato (Lycopersicon esculentum L.) isolated from 2–3 week grown seedlings were cultured in MS liquid medium (2,4-D 1, 6-BA 0.1 mg/l) and fresh medium added subsequently. After 6 weeks culture, the cell clusters were transferred to semisolid medium (the additive same as in liquid medium, agar 0.3%). When the calli grew to 0.5 cm in diameter, transfer them to MS medium (6-BA 2, IAA 0.2 mg/l) for differentiation. The regenerated plants were obtained. After comparing different culture methods, tomato protoplasts grew better in double layers than in agar plate and hanging drops.

Key words: Tomato (Lycopersicon esculentum L.), Protoplasts, Plant regeneration

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