J Integr Plant Biol. ›› 1988, Vol. 30 ›› Issue (5): -.

• Research Articles •    

Cytology and Histology in Somatic Embryogenesis of Indica Rice

Ling Ding-hou, D.S. Brar and F. J. Zapata   

Abstract: The somatic embryogenesis was established from mature dehulled seeds. The histological research showed that embryogenic calli were initiated first from absorbed cells of scutellum of mature seed. And then the embryoids derived from the surface of embryogenic callus. Having been the same structure like a zygotic embryo of rice, the embryoids possessed the major parts of scutellum, coleoptile and coleorhiza. In an embryoid, several developmental stages of pro-embryoid, including single embryogenic cells, two, four and multiple cell stage pro-embryeids and some abnormal embryoids were observed. It could be concluded from this experiment that the embryoid from somatic cell culture in Indica rice possessed an original form of a plant in structure like a zygotic did and derived from a single cell.

Key words: Somatic embryogenesis, Histology of embryoid, Single cell origin of embryoid, lndica rice

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