J Integr Plant Biol. ›› 1989, Vol. 31 ›› Issue (6): -.

• Research Articles •    

Pollen Protoplast Culture Leading to Embryogenic Divisions in Hemerocollis fufva

Zhou Chang   

Abstract: Pollen protoplasts were isolated from the cold-pretreated flower buds containing uninucleate microspores and were inoculated in K3 basic medium supplemented with various additives. A part of the pollen protoplasts were triggered to embryogenic divisions and further to formation of proembryos and clusters. Microscopical observations showed that the first cell division might be equal or unequal and the subsequent growth might be organized or unorganized. This is the first example of pollen protoplast culture in which a sporophytic, or embryogenic, pathway is triggered

Key words: Pollen protoplast, Protoplast culture, Hemerocallis fulva

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