J Integr Plant Biol. ›› 1988, Vol. 30 ›› Issue (4): -.

• Research Articles •    

An Investigation on Relation Between Polysaccharide and Protein in Panax ginseng Pectin

Liang Zhong-yan and Zhang Yi-shen   

Abstract: Two proteoglycans (PA, PB) have been isolated from hot water extract of fresh ginseng roots Through Sepharose 4B gel chromotography it has been determined that both PA and PB are homogeneous fractions but contain proteins. By means of analysis of amino acid, G. C., β-elimination reaction, partial hydrolysis, enzyme hydrolysis, gel electrophoresis and isoelectric focusing electrophoresis it has been determined that both PA and PB are pro- teoglycans and the carbohydratepeptide linkaged by O-glycosidic bond of sugar to threonine Moreover, there is another protein in PB rich in alkaline amino acid (59.9%) combined with the galacturonic acid in polysaccharide by electrostatic force. The protein is dissociated from polysaccharide under the electric-field force in gel electrophoresis. PI of this protein is 9.1.

Key words: Panax ginseng, Pectin, Proteoglycan

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