J Integr Plant Biol. ›› 1988, Vol. 30 ›› Issue (2): -.

• Research Articles •    

Study on the Somatic Embryogenesis from leaf of Begonia fimbristipula Hance in vitro

Zhang Lan-ying, Li Geng-guang and Guo Jun-Yan   

Abstract: Liquid SH medium supplemented with 0.125 mg/l 2,4-D, 0.25–0.50 mg/l BA, and 10% v/v CM or 0.2 w/v CH, incubating on the shaker was favourable to directly initiating normal development and large number of somatic embryoids from leaf of Begonia fambristipula Hance in vitro. Globular, hearttorpedo and cotyledon shaped embryoids appeared in the same medium after incubation for 50 days. Although somatic embryoids and adventitious buds also originated from the epidermal cells of the leaf, there were some differences between them: The development of somatic embryoid involved four different periods, but the adventitions buds had none; Globular embryoid would fall away from explant and distributed in the medium, but buds would remain tightly connected with explant throughout; torpedocotyledon embryoids have the specific structures with shoot and root ends, two cotyledons with the middle apical meristem and a procambium, but the adventitious buds have the shoot apex only.

Key words: Somatic embryogenesis, Begonia firnbristipula

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