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J Integr Plant Biol, 1987, 29 (1): -, Research Article
Dynamics of Newly Synthesized RNA and Protein Diffusion from Germinating Pollen of Cfivia nobilis Cultured In Vitro
Tang Pei-hua and Zhu ying-min
doi:
Abstract
Release of newly synthesized RNA (NSRNA) and protein (NSP) from.germinating pollen(GP) of Clivia nobilis was observed and proven by using autoradiograph, labeled with 3H-uridine and 3H-leucine, and treating with inhibitors (Actinimycin D and Cycloheximide). Its dynamics are as follows: 1 h after labeling before the germination of the pollens, a large amount of NSRNA and NSP were synthesized already. 2 hrs later, the NSP transported towards PT. 5 hrs later, it diffused into the growth medium. The NSRNA .concentrated.in vegetative nucleus and generative cells with in the first 90 min. 2 hrs later it diffused to the protoplasm of the pollens, 3 hrs later-concentrated on the tip of PT. 5 hrs laterout of GP. The biological significance of the phenomenon was discussed.
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