J Integr Plant Biol. ›› 2015, Vol. 57 ›› Issue (6): 550-561.DOI: 10.1111/jipb.12298

• Molecular Physiology • Previous Articles     Next Articles

Nitric oxide alleviates aluminum-induced oxidative damage through regulating the ascorbate-glutathione cycle in roots of wheat

Chengliang Sun1†, Lijuan Liu1†, Yan Yu1, Wenjing Liu1, Lingli Lu1,2, Chongwei Jin1,2 and Xianyong Lin1,2*   

  1. 1MOE Key Laboratory of Environment Remediation and Ecological Health, College of Natural Resource & Environmental Sciences, Zhejiang University, Hangzhou, China
    2Key Laboratory of Subtropical Soil Science and Plant Nutrition of Zhejiang Province, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, China
  • Received:2014-08-09 Accepted:2014-10-12 Published:2014-10-16
  • About author:These authors contributed equally to this work and the paper.
    *Correspondence: E-mail: xylin@zju.edu.cn

Abstract:

The possible association with nitric oxide (NO) and ascorbate-glutathione (AsA-GSH) cycle in regulating aluminum (Al) tolerance of wheat (Triticum aestivum L.) was investigated using two genotypes with different Al resistance. Exposure to Al inhibited root elongation, and triggered lipid peroxidation and oxidation of AsA to dehydroascorbate and GSH to glutathione disulfide in wheat roots. Exogenous NO significantly increased endogenous NO levels, and subsequently alleviated Al-induced inhibition of root elongation and oxidation of AsA and GSH to maintain the redox molecules in the reduced form in both wheat genotypes. Under Al stress, significantly increased activities and gene transcriptional levels of ascorbate peroxidase, glutathione reductase, and dehydroascorbate reductase, were observed in the root tips of the Al-tolerant genotype Jian-864. Nitric oxide application enhanced the activity and gene transcriptional level of these enzymes in both wheat genotypes. γ-Glutamylcysteine synthetase was not significantly affected by Al or NO, but NO treatments increased the activity of glutathione peroxidase and glutathione S-transferase to a greater extent than the Al-treated wheat seedlings. Proline was significantly decreased by Al, while it was not affected by NO. These results clearly suggest that NO protects wheat root against Al-induced oxidative stress, possibly through its regulation of the AsA-GSH cycle.

 

Sun C, Liu L, Yu Y, Liu W, Lu L, Jin C, Lin X (2015) Nitric oxide alleviates aluminum-induced oxidative damage through regulating the ascorbate-glutathione cycle in roots of wheat. J Integr Plant Biol 57: 550–561. doi: 10.1111/jipb.12298

Key words: Aluminum, ascorbate, glutathione, nitric oxide, reactive oxygen species, wheat

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