J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (8): 911-916.DOI: 10.1111/jipb.12785

Special Issue: CRISPR

• Breakthrough Reports • Previous Articles     Next Articles

A strategy for generating rice apomixis by gene editing

En Xie1,2, Yafei Li1, Ding Tang1, Yanli Lv1,2, Yi Shen1 and Zhukuan Cheng1,2*   

  1. 1State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China
    2The University of Chinese Academy of Sciences, Beijing 100049, China

    Email: Zhukuan Cheng (zkcheng@genetics.ac.cn)
  • Received:2019-01-05 Accepted:2019-01-21 Online:2019-01-30 Published:2019-08-01


Apomixis is an asexual reproduction way of plants that can produce clonal offspring through seeds. In this study, we introduced apomixis into rice (Oryza sativa) by mutating OsSPO11‐1, OsREC8, OsOSD1, and OsMATL through a CRISPR/Cas9 system. The quadruple mutant showed a transformation from meiosis to mitosis and produced clonal diploid gametes. With mutated Osmatl, which gives rise to haploid induction in plants, the quadruple mutant is expected to be able to be produced apomictic diploid offspring. We named this quadruple mutant as AOP (Apomictic Offspring Producer) for its ability to produce apomictic offspring.

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