J Integr Plant Biol. ›› 2019, Vol. 61 ›› Issue (6): 765-777.DOI: 10.1111/jipb.12802

• Research Articles • Previous Articles    

Effects of tillage managements and maize straw returning on soil microbiome using 16S rDNA sequencing

Xinyao Xia1, Piaopiao Zhang4, Linlin He3, Xingxing Gao3, Weijun Li3, Yuanyuan Zhou3, Zongxin Li2*, Hui Li3* and Long Yang1*   

  1. 1Agricultural Big-Data Research Center, College of Plant Protection, Shandong Agricultural University, Taian 271018, China
    2Maize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100,China
    3School of Biological and Science Technology, University of Jinan, Jinan 250022, China
    4Department of Clinical, Taishan Medical University, Taian 271016, China

    Email: Long Yang (lyang@sdau.edu.cn, Dr. Yang is fully responsible for the distribution of all materials associated with this article) , Zongxin Li (sdaucliff@sina.com), Hui Li (bio_lih@ujn.edu.cn)
  • Received:2019-02-16 Accepted:2019-03-12 Online:2019-03-26 Published:2019-06-01

Abstract: Agricultural practices could affect bacterial diversity and community structure by altering soil physical and chemical properties. Straw returning and tillage practices are widely used in agriculture, however, the effects of these agricultural practices on microbiomes are still unclear. In the present study, we compared the 18 bacterial communities of soil with different straw returning and tillage treatment combinations. The V3-V4 regions of the 16S ribosomal RNA were amplified and analyzed by high-throughput sequencing technology. The results showed that the bacterial communities were consistently dominated by Acidobacteria, Proteobacteria, Actinobacteria, and Chloroflexi. Short-term straw returning and tillage practices significantly altered the diversity, relative abundance and functions of the soil microbiome. Soil subjected to rotary tillage and straw returning (RTS) combination possessed the highest bacterial diversity and lowest ratio of G+/G- bacteria, indicating that RTS could be an efficient integrated management system to improve microbiome in the short term. Double verifications based on relative abundance and network analysis, revealed close relationships of Mycobacterium and Methylibium with RTS, indicating they could serve as biomarkers for RTS. Investigating microbial changes under different agricultural practices will provide valuable foundations for land sustainable utilization and increase crop yields.

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