J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (6): 812-831.DOI: 10.1111/jipb.12841

Special Issue: Development

• Functional Omics and Systems Biology • Previous Articles     Next Articles

Arabidopsis PEAPODs function with LIKE HETEROCHROMATIN PROTEIN1 to regulate lateral organ growth

Ying Zhu1†, Xiao Luo2†, Xuxin Liu1‡, Wenjuan Wu3, Xiaofeng Cui1, Yuehui He2* and Jirong Huang3*   

  1. 1National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
    2National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China
    3Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai 200234, China

    These authors contributed equally to this work.
    Current address: Xinjiang Agricultural Vocational Technical College.
    Email: Yuehui He (yhhe@sibs.ac.cn); Jirong Huang (huangjr@shnu.edu.cn, Dr. Huang is fully responsible for the distribution of all materials associated with this article)
  • Received:2019-01-30 Accepted:2019-05-13 Online:2019-05-17 Published:2020-06-01


In higher plants, lateral organs are usually of determinate growth. It remains largely elusive how the determinate growth is achieved and maintained. Previous reports have shown that Arabidopsis PEAPOD (PPD) proteins suppress proliferation of dispersed meristematic cells partly through a TOPLESS corepressor complex. Here, we identified a new PPD‐interacting partner, LIKE HETEROCHROMATIN PROTEIN1 (LHP1), using the yeast two‐hybrid system, and their interaction is mediated by the chromo shadow domain and the Jas domain in LHP1 and PPD2, respectively. Our genetic data demonstrate that the phenotype of ppd2 lhp1 is more similar to lhp1 than to ppd2 , indicating epistasis of lhp1 to ppd2 . Microarray analysis reveals that PPD2 and LHP1 can regulate expression of a common set of genes directly or indirectly. Consistently, chromatin immunoprecipitation results confirm that PPD2 and LHP1 are coenriched at the promoter region of their targets such as D3‐TYPE CYCLINS and HIGH MOBILITY GROUP A , which are upregulated in ppd2, lhp1 and ppd2 lhp1 mutants, and that PPD s mediate repressive histone 3 lysine‐27 trimethylation at these loci. Taken together, our data provide evidence that PPD and LHP1 form a corepressor complex that regulates lateral organ growth.

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