J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (2): 393-408.DOI: 10.1111/jipb.13040

Special Issue: Epigenetics Plant-biotic interaction

• Research Articles • Previous Articles     Next Articles

The Class III peroxidase gene OsPrx30, transcriptionally modulated by the AT‐hook protein OsATH1, mediates rice bacterial blight‐induced ROS accumulation

Hao Liu1,2† , Shuangyu Dong1†, Ming Li1†, Fengwei Gu1†, Guili Yang1, Tao Guo1, Zhiqiang Chen1* and Jiafeng Wang1*   

  1. 1National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou 510642, China
    2Guangdong Provincial Key Laboratory of Crop Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

    These authors contributed equally to this work.

    *Correspondences: Zhiqiang Chen (chenlin@scau.edu.cn); Jiafeng Wang (jfwang@scau.edu.cn, Dr. Wang is responsible for the distribution of the materials associated with this article)

  • Received:2020-08-03 Accepted:2020-11-04 Online:2020-11-26 Published:2021-02-01

Abstract: Class III peroxidases (CIII Prxs) play critical roles in plant immunity by scavenging reactive oxygen species (ROS). However, the functions of CIII Prxs in rice (Oryza sativa L.) immunity are largely unexplored. Here, we report a Prx precursor, OsPrx30, that is responsive to the bacterial blight Xanthomonas oryzae pv. oryzae (Xoo). OsPrx30 was primarily expressed in rice roots, leaves, and stems, and its protein product was mainly localized at the endoplasmic reticulum. Overexpression of OsPrx30 enhanced the plant's susceptibility to Xoo by maintaining a high level of peroxidase (POD) activity and reducing the content of H2O2, whereas depletion of OsPrx30 had the opposite effects. Furthermore, we identified an AT‐hook transcription factor, OsATH1, that is specifically bound to the OsPrx30 promoter. As observed in plants overexpressing OsPrx30, depletion of OsATH1 enhanced susceptibility to Xoo. Finally, we demonstrated that depletion of OsATH1 increased histone H3 acetylation at the AT‐rich region of the OsPrx30 promoter. Taken together, these results reveal a mechanism underlying the POD‐induced natural resistance to bacterial diseases and suggest a model for transcription regulation of Prx genes in rice.

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