J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (5): 834-847.DOI: 10.1111/jipb.13049

Special Issue: Development

• Cell and Developmental Biology • Previous Articles     Next Articles

Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development

Mingzhou Yu1†, Mingming Wu1†, Yulong Ren2†, Yihua Wang1†, Jingfang Li1, Cailin Lei2, Yinglun Sun1, Xiuhao Bao1, Hongming Wu1, Hang Yang1, Tian Pan1, Yongfei Wang1, Ruonan Jing1, Mengyuan Yan2, Houda Zhang2, Lei Zhao2, Zhichao Zhao2, Xin Zhang2, Xiuping Guo2, Zhijun Cheng2, Bing Yang1, Ling Jiang1 and Jianmin Wan1,2*   

  1. 1State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
    2National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

    These authors contributed equally to this work.
    *Correspondence: Jianmin Wan (wanjm@njau.edu.cn)
  • Received:2020-08-29 Accepted:2020-12-03 Online:2020-12-07 Published:2021-05-01

Abstract: Pentatricopeptide repeat (PPR) proteins, composing one of the largest protein families in plants, are involved in RNA binding and regulation of organelle RNA metabolism at the post‐transcriptional level. Although several PPR proteins have been implicated in endosperm development in rice (Oryza sativa), the molecular functions of many PPRs remain obscure. Here, we identified a rice endosperm mutant named floury endosperm 18 (flo18) with pleiotropic defects in both reproductive and vegetative development. Map‐based cloning and complementation tests showed that FLO18 encodes a mitochondrion‐targeted P‐type PPR protein with 15 PPR motifs. Mitochondrial function was disrupted in the flo18 mutant, as evidenced by decreased assembly of Complex I in the mitochondrial electron transport chain and altered mitochondrial morphology. Loss of FLO18 function resulted in defective 5′‐end processing of mitochondrial nad5 transcripts encoding subunit 5 of nicotinamide adenine dinucleotide hydrogenase. These results suggested that FLO18 is involved in 5′‐end processing of nad5 messenger RNA and plays an important role in mitochondrial function and endosperm development.

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