J Integr Plant Biol. ›› 2021, Vol. 63 ›› Issue (11): 1982-1994.DOI: 10.1111/jipb.13167

• Plant Reproduction Biology • Previous Articles    

OsEDM2L mediates m6A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation

Kun Ma1,2,3, Jingluan Han1,2,3, Zixu Zhang1,2, Heying Li2, Yanchang Zhao1,2, Qinlong Zhu1,2,3, Yongyao Xie1,2,3, Yao‐Guang Liu1,2,3 and Letian Chen1,2,3*   

  1. 1 State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources, South China Agricultural University, Guangzhou 510642, China
    2 College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    3 Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China

    *Correspondence: Letian Chen (lotichen@scau.edu.cn)
  • Received:2021-08-03 Accepted:2021-08-26 Online:2021-08-27 Published:2021-11-01

Abstract: N6-methyladenosine (m6A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here, we report that the N6-adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE (OsEDM2L) is essential for rice (Oryza sativa L.) anther development. The osedm2l knockout mutant showed delayed tapetal programmed cell death (PCD) and defective pollen development. OsEDM2L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUM DEGENERATION RETARDATION to regulate the expression of ETERNAL TAPETUM 1 (EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2L altered the transcriptomic m6A landscape, and caused a distinct m6A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2L is indispensable for proper messenger RNA m6A modification in rice anther development.

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