J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (1): 167-187.DOI: 10.1111/jipb.13364

• Molecular Physiology • Previous Articles     Next Articles

GmYSL7 controls iron uptake, allocation, and cellular response of nodules in soybean

Xinying Wu1, Yongliang Wang1, Qiaohan Ni1, Haizhen Li1, Xuesong Wu1, Zhanxin Yuan1, Renhao Xiao1, Ziyin Ren1, Jingjing Lu1, Jinxia Yun1, Zhijuan Wang1*, Xia Li1,2*   

  1. 1. National Key Laboratory of Crop Genetic and Improvement, Hubei Hongshan Laboratory, College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 China;
    2. Guangdong Laboratory for Lingnan Modern Agriculture Wushan Road Guangzhou 510642 China
    *Correspondences: Zhijuan Wang (wangzj@mail.hzau.edu.cn); Xia Li (xli@mail.hzau.edu.cn, Dr. Li is responsible for the distribution of the materials associated with this article)
  • Received:2022-04-26 Accepted:2022-09-06 Online:2022-09-15 Published:2023-01-01

Abstract: Iron (Fe) is essential for DNA synthesis, photosynthesis and respiration of plants. The demand for Fe substantially increases during legumes-rhizobia symbiotic nitrogen fixation because of the synthesis of leghemoglobin in the host and Fe-containing proteins in bacteroids. However, the mechanism by which plant controls iron transport to nodules remains largely unknown. Here we demonstrate that GmYSL7 serves as a key regulator controlling Fe uptake from root to nodule and distribution in soybean nodules. GmYSL7 is Fe responsive and GmYSL7 transports iron across the membrane and into the infected cells of nodules. Alterations of GmYSL7 substantially affect iron distribution between root and nodule, resulting in defective growth of nodules and reduced nitrogenase activity. GmYSL7 knockout increases the expression of GmbHLH300, a transcription factor required for Fe response of nodules. Overexpression of GmbHLH300 decreases nodule number, nitrogenase activity and Fe content in nodules. Remarkably, GmbHLH300 directly binds to the promoters of ENOD93 and GmLbs, which regulate nodule number and nitrogenase activity, and represses their transcription. Our data reveal a new role of GmYSL7 in controlling Fe transport from host root to nodule and Fe distribution in nodule cells, and uncover a molecular mechanism by which Fe affects nodule number and nitrogenase activity.

Key words: GmbHLH300, GmYSL7, iron transport, nodule, soybean, symbiosis

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