J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (6): 1408-1422.DOI: 10.1111/jipb.13460

• Cell and Developmental Biology • Previous Articles     Next Articles

ESCRT-III component OsSNF7.2 modulates leaf rolling by trafficking and endosomal degradation of auxin biosynthetic enzyme OsYUC8 in rice

Liang Zhou1,2†, Saihua Chen1,3†, Maohong Cai1, Song Cui1, Yulong Ren2, Xinyue Zhang2, Tianzhen Liu2, Chunlei Zhou1, Xin Jin2, Limin Zhang2, Minxi Wu2, Shuyi Zhang2, Zhijun Cheng2, Xin Zhang2, Cailin Lei2, Qibing Lin2, Xiuping Guo2, Jie Wang2, Zhichao Zhao2, Ling Jiang1, Shanshan Zhu2* and Jianmin Wan1,2*   

  1. 1. State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
    2. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou 225009, China
    These authors contributed equally to this work.
    *Correspondences:Shanshan Zhu(zhushanshan@caas.cn), Jianmin Wan(wanjm@njau.edu.cn, Dr. Wan is fully responsible for the distributions of the materials associated with this article)
  • Received:2022-08-17 Accepted:2023-01-24 Online:2023-01-26 Published:2023-06-01

Abstract: The endosomal sorting complex required for transport (ESCRT) is highly conserved in eukaryotic cells and plays an essential role in the biogenesis of multivesicular bodies and cargo degradation to the plant vacuole or lysosomes. Although ESCRT components affect a variety of plant growth and development processes, their impact on leaf development is rarely reported. Here, we found that OsSNF7.2, an ESCRT-III component, controls leaf rolling in rice (Oryza sativa). The Ossnf7.2 mutant rolled leaf 17 (rl17) has adaxially rolled leaves due to the decreased number and size of the bulliform cells. OsSNF7.2 is expressed ubiquitously in all tissues, and its protein is localized in the endosomal compartments. OsSNF7.2 homologs, including OsSNF7, OsSNF7.3, and OsSNF7.4, can physically interact with OsSNF7.2, but their single mutation did not result in leaf rolling. Other ESCRT complex subunits, namely OsVPS20, OsVPS24, and OsBRO1, also interact with OsSNF7.2. Further assays revealed that OsSNF7.2 interacts with OsYUC8 and aids its vacuolar degradation. Both Osyuc8 and rl17 Osyuc8 showed rolled leaves, indicating that OsYUC8 and OsSNF7.2 function in the same pathway, conferring leaf development. This study reveals a new biological function for the ESCRT-III components, and provides new insights into the molecular mechanisms underlying leaf rolling.

Key words: bulliform cell, leaf rolling, OsSNF7.2, OsYUC8, rice (Oryza sativa), vacuolar degradation

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