J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (8): 1852-1858.DOI: 10.1111/jipb.13509

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Fine-tuning brassinosteroid biosynthesis via 3′UTR-dependent decay of CPD mRNA modulates wood formation in Populus

Dian Wang1†, Xiaoning Hao1†, Li Xu2†, Mengyan Zhao2, Congpeng Wang3, Xihao Yu3, Yingzhen Kong1, Mengzhu Lu4, Gongke Zhou3*, Guohua Chai2* and Xianfeng Tang3*   

  1. 1. College of Agronomy, Qingdao Agricultural University, Qingdao 266109, China;
    2. College of Resources and Environment, Qingdao Agricultural University, Qingdao 266109, China;
    3. College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao 266109, China;
    4. State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China
    These authors contributed equally to this work.
    *Correspondences: Gongke Zhou (zhougk@qau.edu.cn); Guohua Chai (chaigh@qau.edu.cn); and Xianfeng Tang (tangxf@qau.edu.cn, Dr. Tang is fully responsible for the distribution of the materials associated with this article)
  • Received:2023-01-19 Accepted:2023-05-18 Online:2023-05-19 Published:2023-08-01

Abstract: Brassinosteroids (BRs) are plant hormones that regulate wood formation in trees. Currently, little is known about the post-transcriptional regulation of BR synthesis. Here, we show that during wood formation, fine-tuning BR synthesis requires 3′UTR-dependent decay of Populus CONSTITUTIVE PHOTOMORPHOGENIC DWARF 1 (PdCPD1). Overexpression of PdCPD1 or its 3′ UTR fragment resulted in a significant increase of BR levels and inhibited secondary growth. In contrast, transgenic poplars repressing PdCPD1 3′ UTR expression displayed moderate levels of BR and promoted wood formation. We show that the Populus GLYCINE-RICH RNA-BINDING PROTEIN 1 (PdGRP1) directly binds to a GU-rich element in 3′ UTR of PdCPD1, leading to its mRNA decay. We thus provide a post-transcriptional mechanism underlying BRs synthesis during wood formation, which may be useful for genetic manipulation of wood biomass in trees.

Key words: Brassinsteroids biosynthesis, CPD, populus, RNA-BINDING PROTEIN, 3′UTR regulation

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