J Integr Plant Biol. ›› 2008, Vol. 50 ›› Issue (1): 56-61.DOI: 10.1111/j.1744-7909.2007.00590.x

Special Issue: Abiotic Stresses

• Stress & Phytochemistry • Previous Articles     Next Articles

Stress Responsive Zinc-finger Protein Gene of Populus euphratica in Tobacco Enhances Salt Tolerance

Jun-Ying Wang, Xin-Li Xia, Jun-Ping Wang and Wei-Lun Yin   

  • Received:2007-02-12 Accepted:2007-06-27

Abstract: The Populus euphratica stress responsive zinc-finger protein gene PSTZ, which encodes a protein including typical Cys2/His2 zinc finger structure, was isolated by reverse transcription-polymerase chain reaction from P. euphratica. Northern hybridization revealed that its expression was induced under drought and salt stress conditions. To examine its function, cDNA of the PSTZ gene, driven by the cauliflower mosaic virus 35S promoter, was cloned into a plant expression vector pBin438 and introduced into tobacco plants. Transgenic tobacco showed an enhanced salt tolerance, suggesting that PSTZ may play a role in plant responsiveness to salt stress.

Key words: enhance, Populus euphratica, salt tolerance, Stress responsive, zinc-finger protein

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