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Journal of Integrative Plant Biology >

2022 , Vol. 64 >Issue 10: 1856 - 1859

DOI: https://doi.org/10.1111/jipb.13342

Brief Communications

Cas12a-based on-site, rapid detection of genetically modified crops

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  • 1 Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off‐Season Reproduction Regions, Institute of Tropical Bioscience and Biotechnology & San Ya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
    2 Bellagen Biotechnology Co. Ltd., Jinan 250000, China
    3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
    4 Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China
    5 Center for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    6 Hainan Yazhou Bay Seed Lab, Sanya, Hainan 572024, China
    7 Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China

    † These authors contributed equally to the work.
    * Correspondences: Hui Zhao (zhaohui@itbb.org.cn, Dr. Zhao is fully responsible for the distribution of the materials associated with this article); Zhaobo Lang (zblang@psc.ac.cn); Feng Li (lifeng@bellagen.cn)

Received date: 2022-07-17

  Accepted date: 2022-08-10

  Online published: 2022-08-13

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Abstract

A CRISPR/LbCas12a-based nucleic acid detection method that uses crude leaf extracts as samples and is rapid (≤40 min for a full run) and highly sensitive (0.01%) can be used to monitor genetically modified organisms in the field.

Cite this article

Zhiqiang Duan, Xiaoliang Yang, Xingkun Ji, Ying Chen, Xiaomu Niu, Anping Guo, Jian‐Kang Zhu, Feng Li, Zhaobo Lang and Hui Zhao . Cas12a-based on-site, rapid detection of genetically modified crops[J]. Journal of Integrative Plant Biology, 2022 , 64(10) : 1856 -1859 . DOI: 10.1111/jipb.13342

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