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Department of Biological Sciences
University of Calgary, Calgary, Alberta, Canada
E-mail: daekyun.ro@ucalgary.ca
https://profiles.ucalgary.ca/dae-kyun-ro
Area of expertise: plant biochemistry, isoprenoid, phenylpropanoid, metabolic engineering, synthetic biology
Selected Publications:
Utomo, J. C., Barrell, H. B., Kumar, R., Smith, J., Brant, M. S., De la Hoz Siegler, H., and Ro, D. K. (2024). Reconstructing curcumin biosynthesis in yeast reveals the implication of caffeoyl-shikimate esterase in phenylpropanoid metabolic flux. Metab. Eng. 82: 286–296.
Kwon, M., Hodgins, C. L., Salama, E. M., Dias, K. R., Parikh, A., Mackey, A. V., Catenza, K. F., Vederas, J. C., and Ro, D. K. (2023). New insights into natural rubber biosynthesis from rubber-deficient lettuce mutants expressing goldenrod or guayule cis-prenyltransferase. New Phytol. 239: 1098–1111.
Kwon, M., Utomo, J. C., Park, K., Pascoe, C. A., Chiorean, S., Ngo, I., Pelot, K. A., Pan, C. H., Kim, S. W., Zerbe, P., Vederas, J. C., and Ro, D. K. (2022). Cytochrome P450-catalyzed biosynthesis of a dihydrofuran neoclerodane in magic mint (Salvia divinorum). ACS Catalysis 12: 777–782.
Baek, S., Utomo, J. C., Lee, J. Y., Dalal, K., Yoon, Y. J., and Ro, D. K. (2021). The yeast platform engineered for synthetic gRNA-landing pads enables multiple gene integrations by a single gRNA/Cas9 system. Metab. Eng. 64: 111–121.
Xiong, X., Gou, J., Liao, Q., Li, Y., Zhou, Q., Bi, G., Li, C., Du, R., Wang, X., Sun, T., Guo, L., Liang, H., Lu, P., Wu, Y., Zhang, Z., Ro, D. K., Shang, Y., Huang, S., and Yan, J. (2021). The Taxus genome provides insights into paclitaxel biosynthesis. Nat. Plants 7: 1026–1036.
Nguyen, T. D., Kwon, M., Kim, S. U., Fischer, C., and Ro, D. K. (2019). Catalytic plasticity of germacrene A oxidase underlying the diversification of sesquiterpene lactone. Plant Physiol. 181: 945–960.
Qu, Y., Chakrabarty, R., Tran, H. T., Kwon, E. J., Kwon, M., Nguyen, T. D., and Ro, D. K. (2015). A lettuce (Lactuca sativa) homolog of human Nogo-B receptor interacts with cis-prenyltransferase and is necessary for natural rubber biosynthesis. J. Biol. Chem. 290: 1898–1914.
Barriuso, J., Nguyen, D. T., Li, J. W., Roberts, J. N., MacNevin, G., Chaytor, J. L., Marcus, S. L., Vederas, J. C., and Ro, D. K. (2010). Double oxidation of the cyclic nonaketide dihydromonacolin L to monacolin J by a single cytochrome P450 monooxygenase, LovA. J. Am. Chem. Soc. 133: 8078–8081.
Ro, D. K., Paradise, E. M., Ouellet, M., Fisher, K. J., Newman, K. L., Ndungu, J. M., Ho, K. A., Eachus, R. A., Ham, T. S., Kirby, J., Chang, M. C., Withers, S. T., Shiba, Y., Sarpong, R., and Keasling, J. D. (2006). Production of the anti-malarial drug precursor artemisinic acid in engineered yeast. Nature 440: 940–943.
Ro, D. K., Arimura, G., Lau, S. Y., Piers, E., and Bohlmann, J. (2005). Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multi-functional, multi-substrate cytochrome P450 monooxygenase. Proc. Nat. Acad. Sci. U. S. A. 102: 8060–8065.
Selected Registered Patents
Ro, D. K., Newman, K. L., Paradise, E. M., Keasling, J. D., Ouellet, M., Eachus, R. A., Ho, K. A., and Ham, T. S. Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof. US patent, granted in 2012, Patent number: 8163980
Chang, M. C-Y., Eachus, R. A., Ro, D. K., Yasuo, Y., and Keasling, J. D. Nucleic acids encoding modified cytochrome P450 enzymes and methods of use thereof. US patent, granted in 2012, Patent number: 8097438
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