Please wait a minute...
J Integr Plant Biol
Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation in Arabidopsis
Man Cui1,2, Mengjun Gu1,2, Yaru Lu1, Yue Zhang1, Chunlin Chen1, Hong-Qing Ling1,2* and Huilan Wu1*
1The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China
2College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

*Correspondences:
Email: Huilan Wu (hlwu@genetics.ac.cn); Hong-Qing Ling (hqling@genetics.ac.cn, Dr.Ling is fully responsible for the distribution of all materials associated with this article)
doi: 10.1111/jipb.12985
Abstract

Iron is an essential microelement for plant growth. After uptake from the soil, iron is chelated by ligands and translocated from roots to shoots for subsequent utilization. However, the number of ligands involved in iron chelation is unclear. In this study, we identified and demonstrated that GLU1, which encodes a ferredoxin‐dependent glutamate synthase, was involved in iron homeostasis. First, the expression of GLU1 was strongly induced by iron deficiency condition. Second, lesion of GLU1 results in reduced transcription of many iron‐deficiency‐responsive genes in roots and shoots. The mutant plants revealed a decreased iron concentration in the shoots, and displayed severe leaf chlorosis under the condition of Fe limitation, compared to wild‐type. Third, the product of GLU1, glutamate, could chelate iron in vivo and promote iron transportation. Last, we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants. Overall, our results provide evidence that GLU1 is involved in iron homeostasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis .

--------------------------------------------------------------------------------------------------------------------
Received: 03 June 2020      Accepted:    Online on:25 June 2020
This Article
   Full Text  
   Full Text (PDF) 
   Supporting information
Services
   E-mail Link to this Article
   Export Citation for this Article
Citing Articles
   Cited By
Google Scholar
PubMed
Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2020 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
网站备案号:京ICP备16067583号-22