J Integr Plant Biol. ›› 2020, Vol. 62 ›› Issue (12): 1925-1941.DOI: 10.1111/jipb.12985

Special Issue: Nutrition and stress

• Plant-abiotic Interactions • Previous Articles     Next Articles

Glutamate synthase 1 is involved in iron‐deficiency response and long‐distance transportation in Arabidopsis

Man Cui1,2, Mengjun Gu1,2, Yaru Lu1, Yue Zhang1, Chunlin Chen1, Hong‐Qing Ling1,2* and Huilan Wu1*   

  1. 1The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China
    2College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

    Email: Huilan Wu (hlwu@genetics.ac.cn); Hong-Qing Ling (hqling@genetics.ac.cn, Dr.Ling is fully responsible for the distribution of all materials associated with this article)
  • Received:2020-06-03 Accepted:2020-06-22 Online:2020-06-25 Published:2020-12-01


Iron is an essential microelement for plant growth. After uptake from the soil, iron is chelated by ligands and translocated from roots to shoots for subsequent utilization. However, the number of ligands involved in iron chelation is unclear. In this study, we identified and demonstrated that GLU1, which encodes a ferredoxin‐dependent glutamate synthase, was involved in iron homeostasis. First, the expression of GLU1 was strongly induced by iron deficiency condition. Second, lesion of GLU1 results in reduced transcription of many iron‐deficiency‐responsive genes in roots and shoots. The mutant plants revealed a decreased iron concentration in the shoots, and displayed severe leaf chlorosis under the condition of Fe limitation, compared to wild‐type. Third, the product of GLU1, glutamate, could chelate iron in vivo and promote iron transportation. Last, we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants. Overall, our results provide evidence that GLU1 is involved in iron homeostasis through affecting glutamate synthesis under iron deficiency conditions in Arabidopsis.

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