J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (1): 67-75.DOI: 10.1111/j.1744-7909.2005.00002.x

• Research Articles • Previous Articles     Next Articles

Cis- and Trans-Cinnamic Acids Have Different Effects on the Catalytic Properties of Arabidopsis Phenylalanine Ammonia Lyases PAL1, PAL2, and PAL4

Ming-Jie CHEN, Veerappan VIJAYKUMAR, Bing-Wen LU, Bing XIA and Ning LI   

  • Published:2005-01-01

Abstract: Cis-cinnamic acid (CA) is a naturally occurring compound, presumably converted from trans-CA in higher plants. To investigate the effect of cis-CA on the activity of Arabidopsis phenylalanine ammonia lyase (PAL), AtPAL1, AtPAL2, and AtPAL4 genes were isolated using reverse transcription polymerase chain reaction. These genes were fused to a glutathione S-transferase gene and overexpressed in a heterologous prokaryotic system of Escherichia coli. The purified PAL1, PAL2 and PAL4 enzymes were characterized biochemically to determine the effects of cis-CA on the kinetic parameter Km. The results showed that cis-CA is a competitive inhibitor for PAL1, but not PAL2 and PAL4, whereas trans-CA acts as a competitive inhibitor for all three PAL isomers, suggesting that cis- and trans-CA have different effects on the catalytic activity of PAL.

Key words: Arabidopsis thaliana (L.) Heynh., cis-cinnamic acid, enzymatic kinetics, phenylalanine am-monia lyase.

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