J Integr Plant Biol. ›› 2005, Vol. 47 ›› Issue (8): -.DOI: 10.1111/j.1744-7909.2005.00054.x

• Research Articles •    

Isolation of Fucoxanthin from the Rhizoid of Laminaria japonica Aresch

Wen-Jun WANG, Guang-Ce WANG, Ming ZHANG, C. K. TSENG   

Abstract: Fucoxanthin was extracted from the intact rhizoid of Laminaria japonica Aresch with dimethyl sulfoxide (DMSO), and then recovered from the DMSO extract by partitioning into ethyl acetate and subsequent evaporation. Some isolation conditions such as solvent volume and extraction time were screened. The quantity and quality of the extracted fucoxanthin were determined by spectral analysis (absorption spectra and fluorescence emission spectra). The results indicated that: (1) the average total content of fucoxanthin was 122.1 µg in 1 g of fresh L. japonica rhizoid; (2) in comparison with the widely used organic solvent, acetone, DMSO was much more effective for the extraction of fucoxanthin; (3) both DMSO volume and extraction time influenced extraction efficiency such as the recovery rate and purity of fucoxanthin (1 g of fresh L. japonica rhizoid treated with 4 mL DMSO for 60 min, yielded > 88% of the total fucoxanthin with purity 0.63); (4) when (NH4)2SO4 concentration was in the range of 0.5–1.0 mol/L, the pigments rapidly and entirely moved from DMSO into the ethyl acetate phase; (5) the ethyl acetate and DMSO were recycled using a rotary evaporator.

Key words: dimethyl sulfoxide, extraction, fucoxanthin, Laminaria japonica, rhizoid.

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2022 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q