J Integr Plant Biol. ›› 2007, Vol. 49 ›› Issue (3): -.DOI: 10.1111/j.1744-7909.2007.00397.x

• Evolution •    

Genetic Mapping of Laminaria japonica and L. longissima Using Amplified Fragment Length Polymorphism Markers in a “Two-Way Pseudo- Testcross” Strategy

Yuhui Li, Yingxia Yang, Jidong Liu, Xiuliang Wang, Tianxiang Gao1 and Delin Duan   

Abstract: With a ‘’two-way pseudo-testcross’’ mapping strategy, we applied the amplified fragment length polymorphism (AFLP) markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family (Laminaria longissima Aresch. × L. japonica Miyabe) with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1:1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3:1 Mendelian segregation ratio. Among the loci with 1:1 segregating ratios, 79 loci were ordered in 14 linkage groups (648.6 cM) of the paternal map, and 72 loci were ordered in 14 linkage groups (601.9 cM) of the maternal map. The average density of loci was approximately 1 per 8 cM. To investigate the homologies between two parental maps, we used 58 loci segregated 3:1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for Laminaria.

Key words: amplified fragment length polymorphism makers, genetic linkage map, Laminaria japonica, Laminaria longissima, segregation ratio, two-way pseudo-testcross strategy.

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