J Integr Plant Biol. ›› 2006, Vol. 48 ›› Issue (10): 1225-1232.DOI: 10.1111/j.1744-7909.2006.00320.x

• Research Articles •     Next Articles

A Novel Approach to Functional Analysis of the Ribulose Bisphosphate Carboxylase Small Subunit Gene by Agrobacterium-Mediated Gene Silencing

Xiao-Fu Zhou, Peng-Da Ma, Ren-Hou Wang, Bo Liu and Xing-Zhi Wang   

Abstract: A novel approach to virus-induced post-transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunit (rbcS) gene was established and optimized using potato virus X vector and Nicotiana benthamiana as experimental material. The analysis of silencing phenomena, transcriptional level, protein expression, and pigment measurement showed that the expression of the rbcS endogenous gene was inactivated by the expression of a 500-bp homologous cDNA fragment carried in the virus vector. (Author for correspondence. Tel: +86 (0)431 509 9769; E-mail: xingzhi@public.cc.jl.cn)

Key words: Agrobacterium-mediated transient expression, potato virus X, ribulose bisphosphate carboxylase small subunit (rbcS) gene, virus-induced gene silencing.

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