J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (4): 382-392.DOI: 10.1111/j.1744-7909.2009.00819.x

Special Issue: Cell Biology and Functions

• Plant-environmental Interactions • Previous Articles     Next Articles

Overexpressing HRS1 Confers Hypersensitivity to Low Phosphate-Elicited Inhibition of Primary Root Growth in Arabidopsis thaliana

Hong Liu1,2, Huixia Yang1,2, Chongming Wu1,2, Juanjuan Feng1,2, Xin Liu1,Huanju Qin1 and Daowen Wang1*   

  1. 1State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China
    2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • Received:2008-10-21 Accepted:2008-12-25 Published:2009-03-30
  • About author: *Author for correspondence Tel: 86 10 6488 9380; Fax: +86 10 6485 4467; E-mail: dwwang@genetics.ac.cn

Abstract:

Phosphate (Pi) deficiency causes dramatic root system architecture (RSA changes in higher plants. Here we report that overexpression of HRS1 leads to enhanced sensitivity to low Pi-elicited inhibition of primary root growth in Arabidopsis thaliana seedlings. Bioinformatic investigations uncovered that HRS1 and its six homologs encode putative G2-like transcription factors in Arabidopsis. Analysis of promoter::GUS reporter lines revealed that HRS1 transcripts were present mainly in the root hair region and root hair cells under Pi-sufficient conditions. Pi deprivation increased HRS1 expression level and expanded its expression domain. Although HRS1 knockout mutant did not differ from wild type (WT) control irrespective of Pi status, its overexpression lines were significantly more susceptible to low Pi-elicited primary root shortening. In both WT and HRS1 overexpression seedlings, low Pi-induced primary root shortening was accompanied by enhanced root hair cell differentiation, but this enhancement occurred to a greater extent in the latter genotype. Collectively, our data suggest that HRS1 may be involved in the modulation of primary root and root hair growth in Pi-deprived Arabidopsis seedlings, and provide useful clues for further research into the function of HRS1 and its homologs and the mechanisms behind RSA changes under Pi-deficient conditions.

Liu H, Yang H, Wu C, Feng J, Liu X, Qin H, Wang D (2009) Overexpressing HRS1 confers hypersensitivity to low phosphate-elicited inhibition of primary root growth in Arabidopsis thaliana. J. Integr. Plant Biol. 51(4), 382–392.

Key words: Arabidopsis thaliana, G2-like transcription factor, HRS1, phosphate deficiency, root.

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