J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (11): 2421-2436.DOI: 10.1111/jipb.13560

• Abiotic Stress Responses • Previous Articles     Next Articles

Non-specific phospholipase C4 hydrolyzes phosphosphingolipids and phosphoglycerolipids and promotes rapeseed growth and yield

Bao Yang1, Jianwu Li2,3, Jiayu Yan1, Ke Zhang2,3, Zhewen Ouyang1, Yefei Lu1, Huili Wei1, Qing Li1,4, Xuan Yao1,4, Shaoping Lu1, Yueyun Hong1, Xuemin Wang2,3* and Liang Guo1,4*   

  1. 1. National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China;
    2. Department of Biology, University of Missouri, St. Louis, MO 63121, USA;
    3. Donald Danforth Plant Science Center, St. Louis, MO 63132, USA 4. Yazhouwan National Laboratory, Sanya 572025, China
    *Correspondences:Xuemin Wang(swang@danforthcenter.org);Liang Guo(guoliang@mail.hzau.edu.cn, Dr. Guo is fully responsible for the distribution of all materials associated with this article)
  • Received:2023-05-06 Accepted:2023-08-28 Online:2023-08-29 Published:2023-11-01

Abstract: Phosphorus is a major nutrient vital for plant growth and development, with a substantial amount of cellular phosphorus being used for the biosynthesis of membrane phospholipids. Here, we report that NON-SPECIFIC PHOSPHOLIPASE C4 (NPC4) in rapeseed (Brassica napus) releases phosphate from phospholipids to promote growth and seed yield, as plants with altered NPC4 levels showed significant changes in seed production under different phosphate conditions. Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9)-mediated knockout of BnaNPC4 led to elevated accumulation of phospholipids and decreased growth, whereas overexpression (OE) of BnaNPC4 resulted in lower phospholipid contents and increased plant growth and seed production. We demonstrate that BnaNPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in vitro, and plants with altered BnaNPC4 function displayed changes in their sphingolipid and glycerolipid contents in roots, with a greater change in glycerolipids than sphingolipids in leaves, particularly under phosphate deficiency conditions. In addition, BnaNPC4-OE plants led to the upregulation of genes involved in lipid metabolism, phosphate release, and phosphate transport and an increase in free inorganic phosphate in leaves. These results indicate that BnaNPC4 hydrolyzes phosphosphingolipids and phosphoglycerolipids in rapeseed to enhance phosphate release from membrane phospholipids and promote growth and seed production.

Key words: inorganic phosphorus, non-specific phospholipase C4, phosphate deficiency, phospholipids, phosphorus use efficiency, rapeseed

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