J Integr Plant Biol. ›› 2009, Vol. 51 ›› Issue (8): 762-773.DOI: 10.1111/j.1744-7909.2009.00858.x

Special Issue: Sexual Reproductions

• Research Articles • Previous Articles     Next Articles

GNOM-LIKE 2, Encoding an Adenosine Diphosphate-Ribosylation Factor Guanine Nucleotide Exchange Factor Protein Homologous to GNOM and GNL1, is Essential for Pollen Germination in Arabidopsis

Dong-Jie Jia1, Xi Cao1, Wei Wang1, Xiao-Yun Tan1, Xue-Qin Zhang1, Li-Qun Chen1 and De Ye1,2*   

  1. 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
    2 National Center for Plant Gene Research, Beijing 100101, China
  • Received:2009-04-23 Accepted:2009-06-23 Published:2009-08-17
  • About author: *Author for correspondence Tel(Fax): +86 10 6273 4839; E-mail: yede@cau.edu.cn

Abstract:

In flowering plants, male gametes are delivered to female gametophytes by pollen tubes. Although it is important for sexual plant reproduction, little is known about the genetic mechanism that controls pollen germination and pollen tube growth. Here we report the identification and characterization of two novel mutants, gnom-like 2-1 (gnl2-1) and gnl2-2 in Arabidopsis thaliana, in which the pollen grains failed to germinate in vitro and in vivo. GNL2 encodes a protein homologous to the adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factors, GNOM and GNL1 that are involved in endosomal recycling and endoplasmic reticulum-Golgi vesicular trafficking. It was prolifically expressed in pollen grains and pollen tubes. The results of the present study suggest that GNL2 plays an important role in pollen germination.
 

Jia DJ, Cao X, Wang W, Tan XY, Zhang XQ, Chen LQ, Ye D (2009). GNOM-LIKE 2, encoding anadenosine diphosphate-ribosylation factor- guanine nucleotide exchange factor protein homologous to GNOM and GNL1, is essential for pollen germination in Arabidopsis. J. Integr. Plant Biol. 51(8), 762–773.

Key words: adenosine diphosphate-ribosylation factor-guanine nucleotide exchange factor, Arabidopsis, gametophyte, GNL2, male gametophyte-defective, pollen.

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