J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (7): 670-678.DOI: 10.1111/j.1744-7909.2010.00959.x

Special Issue: Sexual Reproductions

• Plant Reproduction Biology • Previous Articles     Next Articles

Identification of gamyb-4 and Analysis of the Regulatory Role of GAMYB in Rice Anther Development

Zhenhua Liu1,2, Wenjie Bao2, Wanqi Liang2, Jingyuan Yin1* and Dabing Zhang2*   

  1. 1School of Life Sciences, Shanghai University, Shanghai 200240, China
    2School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2010-03-07 Accepted:2010-04-08 Published:2010-05-25
  • About author: **Author for correspondence Tel: +86 21 3420 5074; +86 21 2852 1460; fax: +86 21 3420 4869; E-mail:zhangdb@sjtu.edu.cn or jyyin@staff.shu.edu.cn

Abstract:

In higher plants, male reproductive development is a complex biological process that includes cell division and differentiation, cell to cell communication etc., while the mechanism underlying plant male reproductive development remains less understood. GAMYB encodes a gibberellins acid (GA) inducible transcription factor that is required for the early anther development in rice (Oryza sativa L.). Here, we report the isolation and characterization of a new allele gamyb-4 with a C base deletion in the second exon (+2308), causing a frame shift and premature translational termination. Histological analysis showed that gamyb-4 developed abnormal enlarged tapetum and could not undergo normal meiosis. To understand the regulatory role of GAMYB, we carried out quantitative reverse transcription- polymerase chain reaction analysis and comparison of microarray data. These results revealed that the expression of TDR (TAPETUM DEGENERATION RETARDATION), a tapetal cell death regulator, was downregulated in gamyb-4 and udt1 (undeveloped tapetum1). While the GAMYB expression was not obviously changed in tdr and udt1-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development. This work is helpful in understanding the regulatory network in rice anther development.

Liu Z, Bao W, Liang W, Yin J, Zhang D (2010) Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development. J. Integr. Plant Biol. 52(7), 670–678.

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