J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (7): 626-638.DOI: 10.1111/j.1744-7909.2010.00960.x

Special Issue: Plant Signal Transduction

• Plant-environmental Interactions • Previous Articles     Next Articles

The Interactions among DWARF10, Auxin and Cytokinin underlie Lateral Bud Outgrowth in Rice

Shuying Zhang1,5, Gang Li1,5, Jun Fang1, Weiqi Chen2, Haipai Jiang3,Junhuang Zou4, Xue Liu1,5, Xianfeng Zhao1, Xiaobing Li1, Chengcai Chu1, Qi Xie1, Xiangning Jiang2 and Lihuang Zhu1*   

  1. 1The State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    2College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
    3College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China
    4Department of Ophthalmology, University of Utah, Salt Lake City, UT 84132, USA
    5Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2010-01-09 Accepted:2010-04-05 Published:2010-05-27
  • About author:*Author for correspondence Tel: +86 10 6483 6196; Fax: +86 10 6487 3428; E-mail: lhzhu@genetics.ac.cn

Abstract:

Previous studies have shown that DWARF10 (D10) is a rice ortholog of MAX4/RMS1/DAD1, encoding a carotenoid cleavage dioxygenase and functioning in strigolactones/strigolactone-derivatives (SL) biosynthesis. Here we use D10- RNA interference (RNAi) transgenic plants similar to d10 mutant in phenotypes to investigate the interactions among D10, auxin and cytokinin in regulating rice shoot branching. Auxin levels in node 1 of both decapitated D10-RNAi and wild type plants decreased significantly, showing that decapitation does reduce endogenous auxin concentration, but decapitation has no clear effects on auxin levels in node 2 of the same plants. This implies that node 1 may be the location where a possible interaction between auxin and D10 gene would be detected. D10 expression in node 1 is inhibited by decapitation, and this inhibition can be restored by exogenous auxin application, indicating that D10 may play an important role in auxin regulation of SL. The decreased expression of most OsPINs in shoot nodes of D10-RNAi plants may cause a reduced auxin transport capacity. Furthermore, effects of auxin treatment of decapitated plants on the expression of cytokinin biosynthetic genes suggest that D10 promotes cytokinin biosynthesis by reducing auxin levels. Besides, in D10- RNAi plants, decreased storage cytokinin levels in the shoot node may partly account for the increased active cytokinin contents, resulting in more tillering phenotypes.

Zhang S, Li G, Fang J, Chen W, Jiang H, Zou J, Liu X, Zhao X, Li X, Chu C, Xie Q, Jiang X, Zhu L (2010) The interactions among DWARF10, auxin and cytokinin underlie lateral bud outgrowth in rice. J. Integr. Plant Biol. 52(7), 626–638.

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