J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (7): 602-615.DOI: 10.1111/j.1744-7909.2010.00961.x

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology •     Next Articles

Starch Synthesis and Programmed Cell Death during Endosperm Development in Triticale (×Triticosecale Wittmack)

Chun-Yan Li1,2†, Wei-Hua Li1†, Cheng Li1, Denis A. Gaudet2, André Laroche2, Lian-Pu Cao1 and Zhen-Xiang Lu2*   

  1. 1College of Agriculture, Shihezi University, Xinjiang 832003, China
    2Lethbridge Research Centre, Agriculture and Agri-Food Canada, Alberta T1J 4B1, Canada
  • Received:2009-12-22 Accepted:2010-04-12 Published:2010-05-21
  • About author: *Author for correspondence Tel: +86 403 317 3302; Fax: +86 403 382 3156; E-mail: zhen-xiang.lu@agr.gc.ca
    These authors contributed equally to this work.


Triticale (×Triticosecale Wittmack) grains synthesize and accumulate starch as their main energy source. Starch accumulation rate and synthesis activities of ADP-glucose pyrophosphorylase, soluble starch synthases, granule-bound starch synthase and starch-branching enzyme showed similar pattern of unimodal curves during endosperm development. There was no significant difference in activity of the starch granule-bound protein isolated from total and separated starch granules at different developmental stages after anthesis in triticale. Evans Blue staining and analysis of DNA fragmentation indicated that cells of triticale endosperm undergo programmed cell death during its development. Dead cells within the endosperm were detected at 6 d post anthesis (DPA), and evidence of DNA fragmentation was first observed at 21 DPA. The period between initial detection of PCD to its rapid increase overlapped with the key stages of rapid starch accumulation during endosperm development. Cell death occurred stochastically throughout the whole endosperm, meanwhile, the activities of starch biosynthetic enzymes and the starch accumulation rate decreased in the late stages of grain filling. These results suggested that the timing and progression of PCD in triticale endosperm may interfere with starch synthesis and accumulation.

Li CY, Li WH, Li C, Gaudet DA, Laroche A, Cao LP, Lu ZX (2010) Starch synthesis and programmed cell death during endosperm development in triticale (× Triticosecale Wittmack). J. Integr. Plant Biol. 52(7), 602–615.

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