J Integr Plant Biol. ›› 2010, Vol. 52 ›› Issue (10): 933-940.DOI: 10.1111/j.1744-7909.2010.00973.x

• Research Articles • Previous Articles     Next Articles

Evaluation of Protein Extraction Methods for Vitis vinifera Leaf and Root Proteome Analysis by Two-Dimensional Electrophoresis

Neila Jellouli*, Asma Ben Salem, Abdelwahed Ghorbel and Hatem Ben Jouira   

  1. Laboratoire de Physiologie Moléculaire de la Vigne, Centre de Biotechnologie de Borj Cédria, BP 901- Hammam Lif 2050, Tunisia
  • Received:2009-12-08 Accepted:2010-05-27 Published:2010-10-01
  • About author:*Correndence for author Tel: +216 98 937 981; Fax: +216 79 412 638;E-mail: jel_neil@yahoo.fr

Abstract:

An efficient protein extraction method is crucial to ensure successful separation by two-dimensional electrophoresis (2-DE) for recalcitrant plant species, in particular for grapevine (Vitis vinifera L.). Trichloroacetic acid-acetone (TCA-acetone) and phenol extraction methods were evaluated for proteome analysis of leaves and roots from the Tunisian cultivar ‘Razegui’. The phenol-based protocol proved to give a higher protein yield, a greater spot resolution, and a minimal streaking on 2-DE gels for both leaf and root tissues compared with the TCA-based protocol. Furthermore, the highest numbers of detected proteins on 2-DE gels were observed using the phenol extraction from leaves and roots as compared with TCA-acetone extraction.

Jellouli N, Ben Salem A, Ghorbel A, Ben Jouira H (2010) Evaluation of protein extraction methods for Vitis vinifera leaf and root proteome analysis by two-dimensional electrophoresis. J. Integr. Plant Biol. 52(10), 933–940.

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