J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (7): 586-595.DOI: 10.1111/j.1744-7909.2011.01063.x

Special Issue: Rice Genomics and Agriculture

• Research Articles • Previous Articles    

A Transgenic Study on Affecting Potato Tuber Yield by Expressing the Rice Sucrose Transporter Genes OsSUT5Z and OsSUT2M

Aijun Sun1†, Yan Dai1†, Xinsheng Zhang2, Chunmin Li2, Kun Meng1,3, Honglin Xu1, Xiaoli Wei1, Guifang Xiao1, Pieter B.F. Ouwerkerk4, Mei Wang5 and Zhen Zhu1*   

  1. 1National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    2Changli Institute of Pomology, Academy of Hebei Agricultural and Forestry Sciences, Changli 066600, China
    3Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    4Department of Molecular and Developmental Genetics, Institute of Biology (IBL), Leiden University, Clusius Laboratory, Wassenaarseweg RA Leiden, The Netherlands
    5TNO Quality of Life, Zernikedreef 2333 CK Leiden, The Netherlands
  • Received:2011-03-25 Accepted:2011-05-29 Published:2011-07-01
  • About author:*Corresponding author. Tel: +86-10-64852890; Fax +86-10-64852890; E-mail: zzhu@genetics.ac.cn

Abstract:

In many plants, sucrose transporters are essential for both sucrose exports from sources and imports into sinks, indicating a function in assimilate partitioning. To investigate whether sucrose transporters can improve the yield of starch plant, potato plants (Solanum tuberosum L. cv. Désirée) were transformed with cDNAs of the rice sucrose transporter genes OsSUT5Z and OsSUT2M under the control of a tuber-specific, class-I patatin promoter. Compared to the controls, the average fructose content of OsSUT5Z transgenic tubers significantly increased. However, the content of the sugars and starch in the OsSUT2M transgenic potato tubers showed no obvious difference. Correspondingly, the average tuber yield, average number of tubers per plant and average weight of single tuber showed no significant difference in OsSUT2M transgenic tubers with controls. In the OsSUT5Z transgenic lines, the average tuber yield per plant was 1.9-fold higher than the controls, and the average number of tubers per plant increased by more than 10 tubers on average, whereas the average weight of a single tuber did not increase significantly. These results suggested that the average number of tubers per plant showed more contribution than the average weight of a single tuber to the tuber yield per plant.

Sun A, Dai Y, Zhang X, Li C, Meng K, Xu H, Wei X, Xiao G, Ouwerkerk PBF, Wang M, Zhu Z (2011) A transgenic study on affecting potato tuber yield by expressing the rice sucrose transporter genes OsSUT5Z and OsSUT2M. J. Integr. Plant Biol. 53(7), 586–595.

Key words: rice sucrose transporter, OsSUT2M, OsSUT5Z, transgenic potato, tuber yield

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