J Integr Plant Biol. ›› 2011, Vol. 53 ›› Issue (9): 731-742.DOI: 10.1111/j.1744-7909.2011.01066.x

Special Issue: Abiotic Stresses

• Plant-environmental Interactions • Previous Articles     Next Articles

Cloning of a Vacuolar H+-pyrophosphatase Gene from the Halophyte Suaeda corniculata whose Heterologous Overexpression Improves Salt, Saline-alkali and Drought Tolerance in Arabidopsis

Liang Liu1,2†, Ying Wang1†, Nan Wang1, Yuan-Yuan Dong1, Xiu-Duo Fan1,2, Xiu-Ming Liu1, Jing Yang1 and Hai-Yan Li1,2*   

  1. 1Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin 130118, China
    2College of Life Sciences, Jilin Agricultural University, Changchun, Jilin 130118, China
  • Received:2011-02-16 Accepted:2011-06-16 Published:2011-07-15
  • About author:*Corresponding author. Tel: +86 431 8453 3427; Fax: +86 431 8453 3347; E-mail: hyli99@163.com

Abstract:

Salt, saline-alkali conditions, and drought are major environmental factors limiting plant growth and productivity. The vacuolar H+-translocating inorganic pyrophosphatase (V-H+-PPase) is an electrogenic proton pump that translocates protons into vacuoles in plant cells. Expression of V-H+-PPase increases in plants under a number of abiotic stresses, and is thought to have an important role in adaptation to abiotic stress. In this work, we report the isolation and characterization of the gene, ScVP, encoding a vacuolar inorganic pyrophosphatase (V-H+-PPase) from the halophyte, Suaeda corniculata. Semi-quantitative reverse transcription-polymerase chain reaction analysis showed that ScVP was induced in roots, stems and leaves under treatment with salt, saline-alkali and drought. Compared with wild-type (WT) Arabidopsis, transgenic plants overexpressing ScVP accumulated more Na+ in leaves and roots, and showed increased tolerance to high salinity, saline-alkali and drought stresses. The germination percentage of transgenic Arabidopsis seeds was higher than that of WT seeds under the abiotic stresses. The root length of transgenic plants under salt stress was longer than that of WT plants. Furthermore, the rate of water loss during drought stress was higher in WT than in transgenic plants. Collectively, these results indicate that ScVP plays an important role in plant tolerance to salt, saline-alkali and drought stress.

Liu L, Wang Y, Wang N, Dong YY, Fan XD, Liu XM, Yang J, Li HY (2011) Cloning of a vacuolar H+-pyrophosphatase gene from the halophyte Suaeda corniculata whose heterologous overexpression improves salt, saline-alkali and drought tolerance in Arabidopsis. J. Integr. Plant Biol. 53(9), 731–742.

Key words: Drought, H+-pyrophosphatase, Salt, Saline-alkali, Suaeda corniculata

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