J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (5): 462-472.DOI: 10.1111/jipb.12027

• Original Article • Previous Articles     Next Articles

Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize

Panfeng Zhao1, Guobin Zhang1, Xiaojun Wu1, Na Li1, Dianyi Shi1, Dengfeng Zhang2, Chunfang Ji1, Mingliang Xu1 and Shoucai Wang1*   

  1. 1National Maize Improvement Center of China, Key Laboratory of Biology and Genetic Improvement of Maize (Ministry of Agriculture), China Agricultural University, Beijing 100193, China
    2Institute of Crop Science, Chinese Academy of Agricultural Sciences/the National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China
  • Received:2012-10-06 Accepted:2012-12-20 Published:2013-04-11
  • About author:*Corresponding author Tel/Fax: +86 10 6273 2409; E-mail: wangshoucai678@sina.com.cn


Southern rust (Puccinia polysora Underw.) is a major disease that can cause severe yield losses in maize (Zea mays L.). In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 (as the recurrent parent). The 1:1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat (SSR) markers and seven insertion/deletion (InDel) markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat (NBS-LRR) protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.

Zhao P, Zhang G, Wu X, Li N, Shi D, Zhang D, Ji C, Xu M, Wang S (2013) Fine mapping of RppP25, a southern rust resistance gene in maize. J. Integr. Plant Biol. 55(5), 462–472.

Key words: Fine mapping, maize, Puccinia polysora, resistance gene, southern rust

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