J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (11): 1002-1015.DOI: 10.1111/jipb.12089

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

AtFH16, an Arabidopsis Type II Formin, Binds and Bundles both Microfilaments and Microtubules, and Preferentially Binds to Microtubules

Jiaojiao Wang, Yi Zhang, Jing Wu, Lei Meng and Haiyun Ren*   

  1. The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing 100875, China
  • Received:2013-04-15 Accepted:2013-06-13 Published:2013-10-23
  • About author:These authors contributed equally to this research.
    *Corresponding author Tel: +86 10 58806090; Fax: +86 10 58807720; E-mail: hren@bnu.edu.cn

Abstract:

Formins are well-known regulators that participate in the organization of the actin cytoskeleton in organisms. The Arabidopsis thaliana L. genome encodes 21 formins, which can be divided into two distinct subfamilies. However, type II formins have to date been less well characterized. Here, we cloned a type II formin, AtFH16, and characterized its biochemical activities on actin and microtubule dynamics. The results show that the FH1FH2 structure of AtFH16 cannot nucleate actin polymerization efficiently, but can bind and bundle microfilaments. AtFH16 FH1FH2 is also able to bind and bundle microtubules, and preferentially binds microtubules over microfilaments in vitro. In addition, AtFH16 FH1FH2 co-localizes with microtubules in onion epidermal cells, indicating a higher binding affinity of AtFH16 FH1FH2 for microtubules rather than microfilaments in vivo. In conclusion, AtFH16 is able to interact with both microfilaments and microtubules, suggesting that AtFH16 probably functions as a bifunctional protein, and may thus participate in plant cellular processes.

Wang J, Zhang Y, Wu J, Meng L, Ren H (2013) AtFH16, an Arabidopsis type II formin, binds and bundles both microfilaments and microtubules, and preferentially binds to microtubules. J. Integr. Plant Biol. 55(11), 1002–1015.

Key words: Actin filament, Arabidopsis, cytoskeleton, formin, microtubule

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22