J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (4): 350-363.DOI: 10.1111/jipb.12150

• Cell and Developmental Biology • Previous Articles     Next Articles

Auxin biosynthesis by the YUCCA6 flavin monooxygenase gene in woodland strawberry

Hong Liu1,2, Wei-Fa Xie1,2, Ling Zhang1, Victoriano Valpuesta3, Zheng-Wen Ye1, Qing-Hua Gao1 and Ke Duan1,2*   

  1. 1Forestry and Fruit Tree Research Institute, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, China
    2Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Biotechnology, SAAS, Shanghai, China
    3Department of Molecular Biology and Biochemistry, Universidad de Málaga, Málaga 29071, Spain
  • Received:2013-11-19 Accepted:2013-12-13 Published:2013-12-20
  • About author:*Correspondence: E-mail: duanke@saas.sh.cn

Abstract:

Auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry, the reference genomic system for Rosaceae. The mechanisms regulating auxin biosynthesis in strawberry are largely elusive. Recently, we demonstrated that two YUCCA genes are involved in flower and fruit development in cultivated strawberry. Here, we show that the woodland strawberry (Fragaria vesca L.) genome harbors nine loci for YUCCA genes and eight of them encode functional proteins. Transcription pattern in different plant organs was different for all eight FvYUCs. Functionality of the FvYUC6 gene was studied in transgenic strawberry overexpressing FvYUC6, which showed typical high-auxin phenotypes. Overexpression of FvYUC6 also delayed flowering and led to complete male sterility in F. vesca. Additionally, specific repression of FvYUC6 expression by RNA interference significantly inhibited vegetative growth and reduced plant fertility. The development of leaves, roots, flowers, and fruits was greatly affected in FvYUC6-repressed plants. Expression of a subset of auxin-responsive genes was well correlated with the changes of FvYUC6 transcript levels and free indole-3-acetic acid levels in transgenic strawberry. These observations are consistent with an important role of FvYUC6 in auxin synthesis, and support a main role of the gene product in vegetative and reproductive development in woodland strawberry.

Liu H, Xie WF, Zhang L, Valpuesta V, Ye ZW, Gao QH, Duan K (2014) Auxin biosynthesis by the YUCCA6 flavin monooxygenase gene in woodland strawberry. J Integr Plant Biol 56: 350–363. doi: 10.1111/jipb.12150

Key words: Auxin biosynthesis, Fragaria vesca, plant development, RNA interference, YUCCA genes

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