J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (7): 635-647.DOI: 10.1111/jipb.12178

Special Issue: Cell Biology and Functions

• Cell and Developmental Biology • Previous Articles     Next Articles

An auxin-responsive endogenous peptide regulates root development in Arabidopsis

Fengxi Yang1,4†, Yu Song2,3†, Hao Yang1, Zhibin Liu1, Genfa Zhu4 and Yi Yang1*   

  1. 1Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China
    2Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Sciences, Mengla, China
    3Graduate School of the Chinese Academy of Sciences, Beijing, China
    4Floricultural Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong, China
  • Received:2013-11-18 Accepted:2014-01-27 Published:2014-01-30
  • About author:These authors contributed equally to this work.
    *Correspondence: E-mail: yangyi528@scu.edu.cn

Abstract:

Auxin plays critical roles in root formation and development. The components involved in this process, however, are not well understood. Here, we newly identified a peptide encoding gene, auxin-responsive endogenous polypeptide 1 (AREP1), which is induced by auxin, and mediates root development in Arabidopsis. Expression of AREP1 was specific to the cotyledon and to root and shoot meristem tissues. Amounts of AREP1 transcripts and AREP1-green fluorescent protein fusion proteins were elevated in response to indoleacetic acid treatment. Suppression of AREP1 through RNAi silencing resulted in reduction of primary root length, increase of lateral root number, and expansion of adventitious roots, compared to the observations in wild-type plants in the presence of auxin. By contrast, transgenic plants overexpressing AREP1 showed enhanced growth of the primary root under auxin treatment. Additionally, root morphology, including lateral root number and adventitious roots, differed greatly between transgenic and wild-type plants. Further analysis indicated that the expression of auxin-responsive genes, such as IAA3, IAA7, IAA17, GH3.2, GH3.3, and SAUR-AC1, was significantly higher in AREP1 RNAi plants, and was slightly lower in AREP1 overexpressing plants than in wild-type plants. These results suggest that the novel endogenous peptide AREP1 plays an important role in the process of auxin-mediated root development.

 

Yang F, Song Y, Yang H, Liu Z, Zhu G, Yang Y (2014) An auxin‐responsive endogenous peptide regulates root development in Arabidopsis. J Integr Plant Biol 56: 635–647. doi: 10.1111/jipb.12178

Key words: Auxin response, endogenous peptide, root development

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