J Integr Plant Biol. ›› 2023, Vol. 65 ›› Issue (8): 1937-1949.DOI: 10.1111/jipb.13492

• Cell and Developmental Biology • Previous Articles     Next Articles

The F-box protein SHORT PRIMARY ROOT modulates primary root meristem activity by targeting SEUSS-LIKE protein for degradation in rice

Nini Ma1, Nian Li1, Zhongmao Yu1, Chunli Chen1, Dao‐Xiu Zhou1,2 and Yu Zhao1*   

  1. 1. National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Plant Science Paris‐Saclay (IPS2), CNRS, INRAE, University Paris‐Saclay, Orsay 91405, France
    *Correspondence: Yu Zhao (zhaoyu@mail.hzau.edu.cn)
  • Received:2023-01-07 Accepted:2023-04-03 Online:2023-04-04 Published:2023-08-01

Abstract: Root meristem activity is essential for root morphogenesis and adaptation, but the molecular mechanism regulating root meristem activity is not fully understood. Here, we identify an F-box family E3 ubiquitin ligase named SHORT PRIMARY ROOT (SHPR) that regulates primary root (PR) meristem activity and cell proliferation in rice. SHPR loss-of-function mutations impair PR elongation in rice. SHPR is involved in the formation of an SCF complex with the Oryza sativa SKP1-like protein OSK1/20. We show that SHPR interacts with Oryza sativa SEUSS-LIKE (OsSLK) in the nucleus and is required for OsSLK polyubiquitination and degradation by the ubiquitin 26S-proteasome system (UPS). Transgenic plants overexpressing OsSLK display a shorter PR phenotype, which is similar to the SHPR loss-of-function mutants. Genetic analysis suggests that SHPR promotes PR elongation in an OsSLK-dependent manner. Collectively, our study establishes SHPR as an E3 ubiquitin ligase that targets OsSLK for degradation, and uncovers a protein ubiquitination pathway as a mechanism for modulating root meristem activity in rice.

Key words: F-box protein, primary root development, rice, SLK (SEUSS-LIKE), ubiquitination

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