J Integr Plant Biol. ›› 2014, Vol. 56 ›› Issue (11): 1095-1105.DOI: 10.1111/jipb.12209

Special Issue: Sexual Reproductions

• Plant Reproduction • Previous Articles    

PECTATE LYASE-LIKE10 is associated with pollen wall development in Brassica campestris

Jingjing Jiang1,2, Lina Yao1, Youjian Yu1, Meiling Lv1, Ying Miao3 and Jiashu Cao1*   

  1. 1Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, China
    2State Key Laboratory of Agrobiotechnology Shenzhen Base, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China
    3The Center of Molecular Cell and Systems Biology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China
  • Received:2014-02-19 Accepted:2014-04-25 Published:2014-04-29
  • About author:*Correspondence: E-mail: jshcao@zju.edu.cn

Abstract:

PECTATE LYASE-LIKE10 (PLL10) was previously identified as one of the differentially expressed genes both in microspores during the late pollen developmental stages and in pistils during the fertilization process in Chinese cabbage (Brassica campestris ssp. chinensis). Here, antisense-RNA was used to study the functions of BcPLL10 in Chinese cabbage. Abnormal pollen was identified in the transgenic lines (bcpll10-4, -5, and -6). In fertilization experiments, fewer seeds were harvested when the antisense-RNA lines were used as pollen donor. In vivo and in vitro pollen germination assays less germinated pollen tubes were observed in bcpll10 lines. Scanning electron microscopy observation verified that the tryphine materials were over accumulated around the pollen surface and sticked them together in bcpll10. Moreover, transmission electron microscopy observation revealed that the internal endintine was overdeveloped and predominantly occupied the intine, and disturbed the normal proportional distribution of the two layers in the non-germinal furrow region; and no obvious demarcation existed between them in the germinal furrow region in the bcpll10 pollen. Collectively, this study presented a novel PLL gene that played an important role during the pollen wall development in B. campestris, which may also possess potential importance for male sterility usage in agriculture.

 

Jiang J, Yao L, Yu Y, Lv M, Miao Y, Cao J (2014) PECTATE LYASE‐LIKE10 is associated with pollen wall development in Brassica campestris. J Integr Plant Biol 56: 1095–1105. doi: 10.1111/jipb.12209

Key words: Brassica campestris, Brassica rapa, Chinese cabbage, exine, intine, pectate lyase, PLL, pollen wall

Editorial Office, Journal of Integrative Plant Biology, Institute of Botany, CAS
No. 20 Nanxincun, Xiangshan, Beijing 100093, China
Tel: +86 10 6283 6133 Fax: +86 10 8259 2636 E-mail: jipb@ibcas.ac.cn
Copyright © 2021 by the Institute of Botany, the Chinese Academy of Sciences
Online ISSN: 1744-7909 Print ISSN: 1672-9072 CN: 11-5067/Q
备案号:京ICP备16067583号-22