J Integr Plant Biol. ›› 2013, Vol. 55 ›› Issue (8): 710-720.DOI: 10.1111/jipb.12053

Special Issue: Sexual Reproductions

• Plant Reproduction Biology • Previous Articles     Next Articles

The ATP-binding Cassette Transporter OsABCG15 is Required for Anther Development and Pollen Fertility in Rice

Bai-Xiao Niu1‡, Fu-Rong He, Ming He, Ding Ren1, Le-Tian Chen1,2* and Yao-Guang Liu1*   

  1. 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
    2Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
  • Received:2013-01-31 Accepted:2013-03-27 Published:2013-08-01
  • About author:**Corresponding authors: Tel: +86 20 8528 1908; Fax: +86 20 8528 0200; E-mail: ygliu@scau.edu.cn, lotichen@scau.edu.cn
    Co-Editor: Hong Ma
    These authors contributed equally to this work.

Abstract:

Plant male reproductive development is a complex biological process, but the underlying mechanism is not well understood. Here, we characterized a rice (Oryza sativa L.) male sterile mutant. Based on map-based cloning and sequence analysis, we identified a 1,459-bp deletion in an adenosine triphosphate (ATP)-binding cassette (ABC) transporter gene, OsABCG15, causing abnormal anthers and male sterility. Therefore, we named this mutant osabcg15. Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 (meiosis II stage) to stage 10 (late microspore stage). Two genes CYP704B2 and WDA1, involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in osabcg15 mutant, suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther locules. Consistently, histological analysis showed that osabcg15 mutants developed obvious abnormality in postmeiotic tapetum degeneration, leading to rapid degredation of young microspores. The results suggest that OsABCG15 plays a critical role in exine formation and pollen development, similar to the homologous gene of AtABCG26 in Arabidopsis. This work is helpful to understand the regulatory network in rice anther development.

Niu BX, He FR, He M, Ren D, Chen LT, Liu YG (2013) The ATP‐binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice. J. Integr. Plant Biol. 55(8), 710–720.

Key words: ABC-transporter, male sterility, pollen exine, rice (Oryza sativa L.)

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